Tutorials

• Using pycisTopic on human cerebellum single-cell multiome data
  • Downloading data
  • Set up
  • Getting pseudobulk profiles from cell annotations
  • Inferring consensus peaks
  • QC
  • Creating a cisTopic object
  • Adding metadata to a cisTopic object
  • Running scrublet
  • Run models
  • Model selection
  • Clustering and visualization
  • Topic binarization & QC
  • Differentially Accessible Regions (DARs)
  • Save region sets
  • Gene activity
  • Label transfer
  • Exporting to loom